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Blue Oyster Liquid Culture — Amycel 3015
Live Amycel 3015 mycelium in sterile broth, ready to inoculate grain the day it arrives. This is the commercial Pleurotus ostreatus hybrid we run in our own production blocks: fast to colonize, heavy-yielding, and forgiving across a wide range of temperatures.
What You're Getting
One 10 mL syringe of active liquid culture, prepared on a laminar flow hood and inoculated from clean agar. Liquid culture is the fastest way to start grain: the mycelium is already spread through the liquid, so colonization starts from many points at once instead of one wedge of agar. One syringe inoculates about 5–10 lb of grain.
About Our LC Broth
Every culture grows in our house broth, a lean, clear mix of dextrose, light malt extract, soy peptone, and yeast extract in distilled water at about 2%. A richer broth grows faster but makes weaker, more contamination-prone cultures. We keep ours light for vigorous, healthy mycelium. The broth also stays clear, so you can see what you have: healthy LC shows white, wispy clumps in clear liquid. Cloudy or milky broth means bacteria.
Why Amycel 3015?
- Dense, heavy clusters: silver-grey to deep blue-grey caps, 3–8 inches across, with thick flesh and tight margins
- Fruits without a cold shock: produces reliably through spring, summer, fall, and winter, indoors or out
- Not picky about substrate: straw, hardwood sawdust, Master's Mix, supplemented blocks, and coffee grounds all work
- Color you can steer: cooler fruiting with good light brings out the deep blue. Warmer rooms push caps toward pale grey.
How to Use
Liquid culture goes into sterilized grain, not straight into pasteurized straw or bulk substrate.
- Use 1–2 mL per 1 lb of sterilized grain
- Shake the syringe well before each use to break up the mycelium
- Flame the needle until it glows red, let it cool, and wipe the injection port with alcohol before every injection
- Once the grain is fully colonized, use it as spawn for your bulk substrate
Growing Parameters
Incubation temp: 75–82°F
Fruiting temp: 50–75°F (best 55–68°F)
Humidity (pinning): 90–95% RH
Humidity (fruiting): 85–90% RH
Time to first pins: 15–25 days from inoculating bulk substrate
Best substrates: straw, hardwood sawdust, Master's Mix, supplemented blocks
Storage & Handling
- Refrigerate at 35–45°F. Best used within 6–12 months.
- Do not freeze.
- Let it come to room temperature before use.
- Some settling or clumping is normal. Shake it back into suspension.
About Cotaco Creek Mushroom Farm
We're a small commercial mushroom farm in Somerville, Alabama, and every culture we sell is a strain we grow ourselves. Questions about inoculation rates, grain prep, or fruiting conditions? Reach out. We're happy to help.
Blue Oyster Liquid Culture — Amycel 3015
Live Amycel 3015 mycelium in sterile broth, ready to inoculate grain the day it arrives. This is the commercial Pleurotus ostreatus hybrid we run in our own production blocks: fast to colonize, heavy-yielding, and forgiving across a wide range of temperatures.
What You're Getting
One 10 mL syringe of active liquid culture, prepared on a laminar flow hood and inoculated from clean agar. Liquid culture is the fastest way to start grain: the mycelium is already spread through the liquid, so colonization starts from many points at once instead of one wedge of agar. One syringe inoculates about 5–10 lb of grain.
About Our LC Broth
Every culture grows in our house broth, a lean, clear mix of dextrose, light malt extract, soy peptone, and yeast extract in distilled water at about 2%. A richer broth grows faster but makes weaker, more contamination-prone cultures. We keep ours light for vigorous, healthy mycelium. The broth also stays clear, so you can see what you have: healthy LC shows white, wispy clumps in clear liquid. Cloudy or milky broth means bacteria.
Why Amycel 3015?
- Dense, heavy clusters: silver-grey to deep blue-grey caps, 3–8 inches across, with thick flesh and tight margins
- Fruits without a cold shock: produces reliably through spring, summer, fall, and winter, indoors or out
- Not picky about substrate: straw, hardwood sawdust, Master's Mix, supplemented blocks, and coffee grounds all work
- Color you can steer: cooler fruiting with good light brings out the deep blue. Warmer rooms push caps toward pale grey.
How to Use
Liquid culture goes into sterilized grain, not straight into pasteurized straw or bulk substrate.
- Use 1–2 mL per 1 lb of sterilized grain
- Shake the syringe well before each use to break up the mycelium
- Flame the needle until it glows red, let it cool, and wipe the injection port with alcohol before every injection
- Once the grain is fully colonized, use it as spawn for your bulk substrate
Growing Parameters
Incubation temp: 75–82°F
Fruiting temp: 50–75°F (best 55–68°F)
Humidity (pinning): 90–95% RH
Humidity (fruiting): 85–90% RH
Time to first pins: 15–25 days from inoculating bulk substrate
Best substrates: straw, hardwood sawdust, Master's Mix, supplemented blocks
Storage & Handling
- Refrigerate at 35–45°F. Best used within 6–12 months.
- Do not freeze.
- Let it come to room temperature before use.
- Some settling or clumping is normal. Shake it back into suspension.
About Cotaco Creek Mushroom Farm
We're a small commercial mushroom farm in Somerville, Alabama, and every culture we sell is a strain we grow ourselves. Questions about inoculation rates, grain prep, or fruiting conditions? Reach out. We're happy to help.
